Human CASP9 / Caspase 9
(tested or 100% immunogen sequence identity)
Protein G purified
- IHC - Paraffin (10 µg/ml)
- Western blot
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Recombinant human caspase-9 prodomain protein.
Detects an ~46-50 kD protein, corresponding to the apparent molecular mass of the caspase-9 on SDS-PAGE immunoblots, in samples from human and monkey origins. This antibody recognizes the pro-caspase-9 human recombinant protein as well as pro-caspase -9 from human and monkey cell lysates.
Immunohistochemistry: LS-B3672 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3672 was determined to be 10 ug/ml.
PBS, 0.01 mM PMSF, 50% glycerol
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.