Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Human CASP8 / Caspase 8
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IHC - Paraffin (1:1000 - 1:5000)
IHC - Frozen
Western blot (1:1000 - 1:2000)
Immunoprecipitation (1:50 - 1:200)
Specificity and Use
CASP8 / Caspase 8 antibody was raised against peptide sequence corresponding to the C-terminus of human Caspase-8 (PQPTFTLRKKLVFPSD). This sequence is common to various Caspase-8 forms including Caspase 8a (aa481-496), Caspase 8b (aa464-479), Caspase 8c (aa449-464), Caspase8L (aa523-538), Truncated caspase 8 (as251-266). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Marmoset (94%); Orangutan, Mouse, Bovine, Dog, Bat, Elephant, Panda, Horse, Pig (81%).
Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases.
Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using LS-C148246 at 1:2000. A. Anaplastic glioma cores from two different patients, positive (left) and negative (right) staining for caspase-8. B. Higher magnification from the caspase-8 positive (A, left) core.
Requested From: United States
Date Requested: 12/2/2016