Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1) to Human CASP8 / Caspase 8
Western blot, Immunoprecipitation, ELISA
Human CASP8 / Caspase 8
Human (tested or 100% immunogen sequence identity)
Western blot (1:1000)
Specificity and Use
CASP8 / Caspase 8 antibody was raised against synthetic peptide derived from the carboxy-terminal sequence of the p18 fragment of human caspase-8 (KLH).
Recognizes endogenous levels of full length human caspase-8 (57kD) as well as the cleaved intermediate p43/p41 and the caspase-8 active subunit p18. This antibody does not cross-react with other caspases.
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:100.
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol, 0.02% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases.