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Anti-CASP8 / Caspase 8 Antibody (aa217-222, clone 90A992) LS-C507

Ordering

Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CASP8 / Caspase 8 Antibodies

Anti-Ephrin B, pan Antibody LS-C23124
Mouse Monoclonal (IgG1,k)
Mouse
Western blot, Immunoprecipitation, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 90A992] (IgG1) to Human CASP8 / Caspase 8
Human, Monkey, Mouse
Western blot, Flow Cytometry
Unconjugated

Details

Human CASP8 / Caspase 8
Mouse
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [90A992]
Protein G purified

Applications

Western blot (0.5 - 2 µg/ml), Flow Cytometry
IHC - Paraffin

Specificity and Use

CASP8 / Caspase 8 antibody was raised against amino acids PVETDSEEQPof human Caspase-8 were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mosquito, Nematode, Arabidopsis, Coccidioides (80%).
aa217-222
This can be used for detection of the pro-form of Caspase-8.

Packaging

PBS, 0.02% sodium azide.
Long term: -70°C; Short term: +4°C
For research use only.

About CASP8 / Caspase 8

Q14790 nm_001228 AAC50645.1

CASP8 Antibody, ALPS2B Antibody, Apoptotic protease Mch-5 Antibody, Apoptotic cysteine protease Antibody, Casp-8 Antibody, Caspase-8 Antibody, CAP4 Antibody, Caspase 8 Antibody, MACH Antibody, MCH5 Antibody, MORT1-associated ced-3 homolog Antibody, ICE-like apoptotic protease 5 Antibody, Pro Caspase 8 Antibody, FADD-like ICE Antibody, FLICE Antibody, MACH-alpha-1/2/3 protein Antibody, MACH-beta-1/2/3/4 protein Antibody

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases.

Western blot

Western blot
Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.

Flow Cytometry

Flow Cytometry
Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.

Requested From: United States
Date Requested: 9/30/2016

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