Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 90A992] (IgG1) to Human CASP8 / Caspase 8
Human, Monkey, Mouse
Western blot, Flow Cytometry
Human CASP8 / Caspase 8
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [90A992]
Protein G purified
Western blot (0.5 - 2 µg/ml)
IHC - Paraffin
Specificity and Use
CASP8 / Caspase 8 antibody was raised against amino acids PVETDSEEQPof human Caspase-8 were used as the immunogen for this antibody. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mosquito, Nematode, Arabidopsis, Coccidioides (80%).
This can be used for detection of the pro-form of Caspase-8.
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases.
Western blot analysis for human Caspase-8 using Jurkat lysates with antibody at 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). antibody only detects 55 kD Caspase-8 in Jurkat cells.
Flow cytometry of Caspase-8 in HeLa cells using 0.1 ug of Caspase-8 antibody. Shaded histogram represents cells without antibody; green represents isotype control, ; red represents Caspase-8 antibody. Goat anti-mouse IgG-FITC secondary antibody, was used for this test.