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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASP7 / Caspase 7 Antibody (clone 25B881.1) IHC‑plus™ LS‑B2020
Note: This antibody replaces LS-C506
Caspase 7 antibody LS-B2020 is an unconjugated mouse monoclonal antibody to human Caspase 7 (CASP7). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2020-50
50 µg (0.5 mg/ml)
Unavailable

Popular CASP7 / Caspase 7 Products

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Product Description

Caspase 7 antibody LS-B2020 is an unconjugated mouse monoclonal antibody to human Caspase 7 (CASP7). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CASP7 / Caspase 7
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. P55210 NM_001227 NP_001218.1

CASP7 Antibody, Apoptotic protease Mch-3 Antibody, Caspase 7 Antibody, Caspase-7 Antibody, ICE-LAP3 Antibody, LICE2 Antibody, MCH3 Antibody, ICE-like apoptotic protease 3 Antibody, CASP-7 Antibody, CMH-1 Antibody


Specifications

Target
Human CASP7 / Caspase 7
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [25B881.1]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human Caspase-7.
Specificity
In Jurkat, a 35 kD band is observed.
Usage
Immunohistochemistry: LS-B2020 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2020 was determined to be 10 ug/ml.
Presentation
PBS containing 0.2% gelatin and 0.05% sodium azide.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2020 concentration 10 ug/ml.
Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2020 concentration 10 ug/ml.

Western blot

Western blot of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.
Western blot of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.

Western blot

Western blot analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.
Western blot analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.

Immunohistochemistry

Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2020 concentration 10 ug/ml.
Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2020 concentration 10 ug/ml.

Western blot

Western blot of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.
Western blot of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.

Western blot

Western blot analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.
Western blot analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.

Immunohistochemistry

Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2020 concentration 10 ug/ml.
Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2020 concentration 10 ug/ml.

Western blot

Western blot of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.
Western blot of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.

Western blot

Western blot analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.
Western blot analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.

Immunohistochemistry

Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2020 concentration 10 ug/ml.
Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2020 concentration 10 ug/ml.

Western blot

Western blot of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.
Western blot of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.

Western blot

Western blot analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.
Western blot analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.


Requested From: 
Date Requested: 6/25/2018

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