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Anti-CASP7 / Caspase 7 Antibody (clone 25B881.1) IHC-plus™ LS-B2020

Note: This antibody replaces LS-C506

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CASP7 / Caspase 7 Antibodies

Anti-CASP7 / Caspase 7 Antibody (N-Terminus) IHC-plus™ LS-B3795
Rabbit Polyclonal to Human CASP7 / Caspase 7
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CASP7 / Caspase 7 Antibody (aa107-156) LS-C135480
Rabbit Polyclonal to Human CASP7 / Caspase 7
Human, Monkey, Mouse, Rat, Dog, Horse, Pig, Zebrafish
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 25B881.1] (IgG1) to Human CASP7 / Caspase 7
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human CASP7 / Caspase 7
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [25B881.1]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 2 µg/ml)

Specificity and Use

CASP7 / Caspase 7 antibody was raised against recombinant human Caspase-7.
In Jurkat, a 35 kD band is observed.
Immunohistochemistry: LS-B2020 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2020 was determined to be 10 ug/ml.

Packaging

PBS containing 0.2% gelatin and 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CASP7 / Caspase 7

P55210 NM_001227 NP_001218.1

CASP7 Antibody, Apoptotic protease Mch-3 Antibody, Caspase 7 Antibody, Caspase-7 Antibody, ICE-LAP3 Antibody, LICE2 Antibody, MCH3 Antibody, ICE-like apoptotic protease 3 Antibody, CASP-7 Antibody, CMH-1 Antibody

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens.

Immunohistochemistry

Immunohistochemistry
Anti-Caspase 7 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2020 concentration 10 ug/ml.

Western blot

Western blot
Western blot of Caspase-7 in A) human brain, B) mouse brain and C) rat brain lysate using Caspase-7 antibody at 1 ug/ml.

Western blot

Western blot
Western blot analysis of Caspase-7 in Jurkat cells using Caspase-7 antibody at 1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. Caspase-7 activation is detected in western blots by the presence of Caspase-7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) Caspase-7, depending on the treatment time points. A basal level of endogenous intermediate Caspase-7 can be see in untreated Jurkat cells.
 

Requested From: United States
Date Requested: 12/4/2016

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