Human CASP3 / Caspase 3

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Human full-length Caspase 3 fusion protein containing a histidine-6 tag.

Recognizes full-length Caspase 3 (Yama/Apopain) at 32kD and proteolytic fragments as demonstrated after in vitro cleavage of recombinant Caspase 3. Cross-reacts with human, mouse and rat.

• Western blot (0.5 - 2 µg/ml)

Suitable for use in Western Blot. Western Blot: 0.5-2 ug/ml (will detect the 32kD precursor of Caspase 3 in RIPA lysates from non-stimulated human A431 cells; previous lots detected Caspase 3 in RIPA lysates of mouse 3T3 and rat PC12 cells). Use 20 ug per lane for minigels. Positive Antigen Control: non-stimulated A431 cell lysate. Western Blot Analysis: Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Caspase 3 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

0.07 M Tris-Glycine, pH 7.4, 0.105 M NaCl, 0.035% Sodium Azide, 30% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P42574 NCBI: NM_004346 NP_004337.2