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Anti-CASP3 / Caspase 3 Antibody LS-C12481

Ordering

Wt. Vol. Conc. Price
200 µg - - $590
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Rabbit Polyclonal (IgG) to Human CASP3 / Caspase 3
Human, Mouse, Rat
Western blot
Unconjugated

Details

Human CASP3 / Caspase 3
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified

Applications

Western blot (0.5 - 2 µg/ml)

Specificity and Use

CASP3 / Caspase 3 antibody was raised against human full-length Caspase 3 fusion protein containing a histidine-6 tag.
Recognizes full-length Caspase 3 (Yama/Apopain) at 32kD and proteolytic fragments as demonstrated after in vitro cleavage of recombinant Caspase 3. Cross-reacts with human, mouse and rat.
Suitable for use in Western Blot. Western Blot: 0.5-2 ug/ml (will detect the 32kD precursor of Caspase 3 in RIPA lysates from non-stimulated human A431 cells; previous lots detected Caspase 3 in RIPA lysates of mouse 3T3 and rat PC12 cells). Use 20 ug per lane for minigels. Positive Antigen Control: non-stimulated A431 cell lysate. Western Blot Analysis: Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Caspase 3 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Packaging

0.07 M Tris-glycine, pH 7.4, 0.105 M sodium chloride, 0.035% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CASP3 / Caspase 3

P42574 NM_004346 NP_004337.2

CASP3 Antibody, Apopain Antibody, CASP-3 Antibody, Caspase-3 Antibody, CPP32B Antibody, CPP32 Antibody, Cysteine protease CPP32 Antibody, SCA-1 Antibody, SREBP cleavage activity 1 Antibody, Yama Antibody, Procaspase3 Antibody, Caspase 3 Antibody, CPP-32 Antibody, PARP cleavage protease Antibody, Protein Yama Antibody

CASP3 / Caspase 3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme.

Requested From: United States
Date Requested: 9/26/2016

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