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Anti-CASP3 / Caspase 3 Antibody (N-Terminus) LS-C12477

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Rabbit Polyclonal (IgG) to Human CASP3 / Caspase 3
Human
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human CASP3 / Caspase 3
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Tissue culture supernatant

Applications

IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot (1:1000), Immunoprecipitation (1:50), Flow Cytometry (1:200)

Specificity and Use

CASP3 / Caspase 3 antibody was raised against synthetic peptide (KLH coupled) corresponding to amino-terminal residues adjacent to (Asp175) in human caspase-3.
N-Terminus
Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases.
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunohistochemistry (Frozen): 1:200 using 3% formaldehyde as fixative. Immunohistochemistry (Paraffin): 1:200. Antigen retrieval: heat in citrate buffer, pH 8.0. Immunofluorescence (IC): 1:200. Flow Cytometry: 1:200.

Packaging

10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CASP3 / Caspase 3

P42574 NM_004346 NP_004337.2

CASP3 Antibody, Apopain Antibody, CASP-3 Antibody, Caspase-3 Antibody, CPP32B Antibody, CPP32 Antibody, Cysteine protease CPP32 Antibody, SCA-1 Antibody, SREBP cleavage activity 1 Antibody, Yama Antibody, Procaspase3 Antibody, Caspase 3 Antibody, CPP-32 Antibody, PARP cleavage protease Antibody, Protein Yama Antibody

CASP3 / Caspase 3 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme.

Requested From: United States
Date Requested: 7/4/2015

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