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Anti-CASP3 / Caspase 3 Antibody (N-Terminus) LS-C12477

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Rabbit Polyclonal (IgG) to Human CASP3 / Caspase 3
Human
IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated

Details

Human CASP3 / Caspase 3
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Tissue culture supernatant

Applications

IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot (1:1000), Immunoprecipitation (1:50), Flow Cytometry (1:200)

Specificity and Use

CASP3 / Caspase 3 antibody was raised against synthetic peptide (KLH coupled) corresponding to amino-terminal residues adjacent to (Asp175) in human caspase-3.
N-Terminus
Detects endogenous levels of the large fragment (17/19kD) of activated caspase-3 resulting from cleavage adjacent to Asp175. Does not recognize full length caspase-3 or other cleaved caspases.
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunohistochemistry (Frozen): 1:200 using 3% formaldehyde as fixative. Immunohistochemistry (Paraffin): 1:200. Antigen retrieval: heat in citrate buffer, pH 8.0. Immunofluorescence (IC): 1:200. Flow Cytometry: 1:200.

Packaging

10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About CASP3 / Caspase 3

P42574 NM_004346 NP_004337.2

CASP3 Antibody, Apopain Antibody, CASP-3 Antibody, Caspase-3 Antibody, CPP32B Antibody, CPP32 Antibody, Cysteine protease CPP32 Antibody, SCA-1 Antibody, SREBP cleavage activity 1 Antibody, Yama Antibody, Procaspase3 Antibody, Caspase 3 Antibody, CPP-32 Antibody, PARP cleavage protease Antibody, Protein Yama Antibody

Caspase 3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase 3 proteolytically cleaves and activates other proteins. Caspase 3 has been associated with apoptosis.

Requested From: United States
Date Requested: 3/31/2015

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