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Anti-CASP2 / Caspase 2 Antibody (N-Terminus) LS-C18764

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CASP2 / Caspase 2 Antibodies

Anti-CASP2 / Caspase 2 Antibody (N-Terminus) IHC-plus™ LS-B475
Rabbit Polyclonal to Human CASP2 / Caspase 2
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CASP2 / Caspase 2 Antibody IHC-plus™ LS-B5926
Rabbit Polyclonal (IgG2b,k) to Human CASP2 / Caspase 2
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-CASP2 / Caspase 2 Antibody (aa123-172) IHC-plus™ LS-B7657
Rabbit Polyclonal (IgG) to Human CASP2 / Caspase 2
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Rat CASP2 / Caspase 2
Rat, Mouse
Western blot, ELISA
Unconjugated

Details

Rat CASP2 / Caspase 2
Rabbit
Rat, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot
  • ELISA (1:1500 - 1:6000)

Specificity and Use

CASP2 / Caspase 2 antibody was raised against synthetic peptide from rat CASP2 / Caspase 2.
N-Terminus
A the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.
This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kD mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2. No reaction was observed against human caspase-2. Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2. This product was assayed against; 1.0 ug of immunizing peptide in a standard sandwich ELISA; using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884 and TMB as a substrate for 30 minutes at room temperature. A 1:750 dilution is recommended for immunoblot.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store vial at -20 C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 C or below.
For research use only.

About CASP2 / Caspase 2

P42575 NM_032982 NP_116764.2

CASP2 Antibody, CASP-2 Antibody, Caspase-2 Antibody, PPP1R57 Antibody, Protease ICH-1 Antibody, NEDD-2 Antibody, Caspase 2 Antibody, ICH1 Antibody, NEDD2 Antibody

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1b and IL-18.

Western blot

Western blot
Anti-Caspase-2 Antibody - Western Blot. Affinity Purified antibody to Caspase-2 was used at a 1:750 dilution to detect rat caspase-2 in transfected human 292 cell lysates by Western blot. Asterisk indicates a 48 kD caspase-2 protein clearly detected in the rat caspase-2 transfected lysate. No reaction is observed against human caspase-2. Control (lane 1) and transfected (lane 2) lysates were loaded on a 4-20% gel for SDS-PAGE. After primary antibody incubation and washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results.
 

Requested From: United States
Date Requested: 12/3/2016

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