Rat CASP2 / Caspase 2

Rabbit

Rat, Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.

N-Terminus

The antibody detects long and short forms of the protein. Antiserum was purified against the immunizing peptide. This antibody reacts with mouse and rat caspase-2, but not caspase-2 from human sources. Cross reactivity with caspase-2 from other sources has not been determined. Rat and mouse caspase-2 show 94% homology. There is no significant homology to the human form.

• Western blot (1:750)
• ELISA (1:1500 - 1:6000)

This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kD mouse and rat caspase-2. A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2. No reaction was observed against human caspase-2. Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2. This product was assayed against; 1.0 ug of immunizing peptide in a standard sandwich ELISA; using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884 and TMB as a substrate for 30 minutes at room temperature. A 1:750 dilution is recommended for immunoblot.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P42575 NCBI: NM_032982 NP_116764.2