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Anti-CASP10 / Caspase 10 Antibody (aa281-330) LS-C116826

Catalog Size Price
LS-C116826-50 50 µg Unavailable

Most Popular CASP10 / Caspase 10 Antibodies

Anti-CASP10 / Caspase 10 Antibody (C-Terminus) LS-C50302
Rabbit Polyclonal to Human CASP10 / Caspase 10
Human
IHC
Unconjugated
Human liver ca. stained with anti-Caspase 10
Anti-CASP10 / Caspase 10 Antibody (C-Terminus) LS-C351929
Rabbit Polyclonal to Human CASP10 / Caspase 10
Human, Mouse, Rat
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of Caspase 10 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-CASP10 / Caspase 10 Antibody (aa400-480) LS-C382445
Rabbit Polyclonal (IgG) to Human CASP10 / Caspase 10
Human
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot of Caspase-10 B/C antibody
Anti-CASP10 / Caspase 10 Antibody (aa223-459, Biotin) LS-C685518
Rabbit Polyclonal (IgG) to Human CASP10 / Caspase 10
Human, Mouse
IHC, Western blot
Biotin Conjugated
Western blot of CASP10 / Caspase 10 antibody.
Anti-CASP10 / Caspase 10 Antibody (aa223-459, FITC) LS-C692008
Rabbit Polyclonal (IgG) to Human CASP10 / Caspase 10
Human, Mouse
IHC, Western blot
FITC Conjugated
Western blot of CASP10 / Caspase 10 antibody.

100% Guaranteed
Rabbit Polyclonal to Human CASP10 / Caspase 10
Human
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human CASP10 / Caspase 10
Rabbit
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

Synthetic peptide located between aa281-330 of human CASP10 (Q92851, NP_001221). Percent identity by BLAST analysis: Human, Gorilla (100%); Orangutan, Gibbon, Monkey (92%).
LS-E21590 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C116826. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa281-330
Human CASP10 / Caspase 10
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
For research use only.

About CASP10 / Caspase 10

Q92851 NM_001230 NP_001221.2

CASP10 Antibody, ALPS2 Antibody, Caspase-10 Antibody, Caspase 10 Antibody, CASP-10 Antibody, FADD-like ICE2 Antibody, FLICE2 Antibody, MCH4 Antibody, ICE-like apoptotic protease 4 Antibody, Apoptotic protease Mch-4 Antibody

CASP10 / Caspase 10 is a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8.

Western blot

CASP10 / Caspase 10 antibody LS-C116826 Western blot of COLO205 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CASP10 / Caspase 10 antibody LS-C116826 Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/22/2018

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