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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cardiolipin Synthase / CRLS1 Antibody (aa161‑174) IHC‑plus™ LS‑B9167
Note: This antibody replaces LS-C154873
CRLS1 antibody LS-B9167 is an unconjugated goat polyclonal antibody to human CRLS1 (Cardiolipin Synthase). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9167-50
50 µg (0.5 mg/ml)
Unavailable

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Human Tonsil, Sketetal Myocytes: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
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Species: Human
Applications: IHC, Western blot, ELISA

Product Description

CRLS1 antibody LS-B9167 is an unconjugated goat polyclonal antibody to human CRLS1 (Cardiolipin Synthase). Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About Cardiolipin Synthase / CRLS1
Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol. Q9UJA2 NM_019095 NP_061968.1

CRLS1 Antibody, Cardiolipin synthase Antibody, C20orf155 Antibody, Cardiolipin synthase 1 Antibody, CLS1 Antibody, DJ967N21.6 Antibody, GCD10 Antibody, CLS Antibody, Protein GCD10 homolog Antibody


Specifications

Target
Human Cardiolipin Synthase / CRLS1
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 0.6 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide C-RSALGSALDPLADK from an internal region of human CRLS1 (NP_061968.1; NP_001120930.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Dog, Bovine, Rabbit, Horse, Porcine, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Salmon, Smelt, Medaka, Pufferfish, Zebrafish (93%); Seq squirt, Capsaspora, Spikemoss (86%).
Blocking Peptide
LS-E27745 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9167. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa161-174
Specificity
Human CRLS1. This antibody is expected to recognize both reported isoforms (NP_061968.1; NP_001120930.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 30kD band observed in lysates of cell line HEK293 (calculated MW of 32.6kD according to NP_061968.1). Recommended concentration: 0.2-0.6 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-Cardiolipin Synthase / CRLS1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Cardiolipin Synthase / CRLS1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

CRLS1 antibody (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CRLS1 antibody (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-Cardiolipin Synthase / CRLS1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Cardiolipin Synthase / CRLS1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

CRLS1 antibody (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CRLS1 antibody (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-Cardiolipin Synthase / CRLS1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Cardiolipin Synthase / CRLS1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

CRLS1 antibody (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CRLS1 antibody (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-Cardiolipin Synthase / CRLS1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Cardiolipin Synthase / CRLS1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

CRLS1 antibody (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CRLS1 antibody (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/23/2018

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