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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CARD8 / Cardinal / TUCAN Antibody (C‑Terminus) IHC‑plus™ LS‑B472 Best Seller
Note: This antibody replaces LS-C338
TUCAN antibody LS-B472 is an unconjugated rabbit polyclonal antibody to human TUCAN (CARD8 / Cardinal). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B472-50
50 µg (1 mg/ml)
Unavailable

Popular CARD8 / Cardinal / TUCAN Products

Western blot of CARD8 / TUCAN antibody LS-C48643.
Species: Human, Mouse, Rat
Applications: Western blot
Antibody
Species: Human
Applications: ICC, Western blot, Immunoprecipitation, Flow Cytometry
Formalin-fixed, paraffin-embedded tumor/normal adjacent tissue cores from a human colontissue microarray stained for TUCAN/CARD8 expression at 1:2000 using LS-C148305. A and B are tumor tissue cores. A1 and B1 are the matched normal adjacent cores from A and B, respectively. Hematoxylin-Eos in counterstain.
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CARD8 mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, Dot Blot

Product Description

TUCAN antibody LS-B472 is an unconjugated rabbit polyclonal antibody to human TUCAN (CARD8 / Cardinal). Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About CARD8 / Cardinal / TUCAN
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. Q9Y2G2 NM_014959 NP_055774.2

CARD8 Antibody, Apoptotic protein NDPP1 Antibody, DAKAR Antibody, KIAA0955 Antibody, NDPP Antibody, TUCAN Antibody, CARDINAL Antibody, DACAR Antibody, NDPP1 Antibody


Specifications

Target
Human CARD8 / Cardinal / TUCAN
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30031 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B472. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Usage
Immunohistochemistry: LS-B472 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B472 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CARD8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B472 concentration 5 ug/ml.
Anti-CARD8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B472 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 µg/mL.
Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 ug/ml.
Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 µg/mL.
Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 ug/ml.
Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 ug/ml.

Western blot

Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 µg/ml.
Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 µg/ml.

Western blot

Western blot of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 ug /ml.
Western blot of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 ug /ml.

Immunohistochemistry

Anti-CARD8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B472 concentration 5 ug/ml.
Anti-CARD8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B472 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 µg/mL.
Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 ug/ml.
Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 µg/mL.
Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 ug/ml.
Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 ug/ml.

Western blot

Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 µg/ml.
Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 µg/ml.

Western blot

Western blot of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 ug /ml.
Western blot of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 ug /ml.

Immunohistochemistry

Anti-CARD8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B472 concentration 5 ug/ml.
Anti-CARD8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B472 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 µg/mL.
Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 ug/ml.
Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 µg/mL.
Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 ug/ml.
Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 ug/ml.

Western blot

Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 µg/ml.
Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 µg/ml.

Western blot

Western blot of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 ug /ml.
Western blot of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 ug /ml.

Immunohistochemistry

Anti-CARD8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B472 concentration 5 ug/ml.
Anti-CARD8 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B472 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 µg/mL.
Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 µg/mL.

Immunocytochemistry

Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 ug/ml.
Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 µg/mL.
Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 ug/ml.
Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 ug/ml.

Western blot

Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 µg/ml.
Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 µg/ml.

Western blot

Western blot of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 ug /ml.
Western blot of CARD8 expression in K562 cell lysate with CARD8 antibody at (A) 2 and (B) 4 ug /ml.


Requested From: 
Date Requested: 6/17/2018

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