Yeast Carboxypeptidase Y

Rabbit

Yeast (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Ion exchange chromatography

Unmodified

Carboxypeptidase Y (yeast).

Immunoelectrophoretic assay gives a single precipitin arc against anti-rabbit serum, purified carboxypeptidase Y and partially purified carboxypeptidase Y (yeast). Although not tested, the antibody should exhibit cross-reactivity with other tissues and species.

• Western blot (1:2500)
• Immunoprecipitation
• ELISA (1:10000 - 1:42000)

Suitable for use in Western Blot (Western or Dot Blot), ELISA, Immunoprecipitation and Conjugation. ELISA: 1:10000-1:42000. Western Blot: 1:2500. Both the antiserum and IgG fractions of anti-carboxypeptidase Y (Baker's yeast) are shown to detect under reducing conditions of SDS-PAGE the 61kD enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5000 dilution of the primary antibody is used followed by detection using HRP goat-a-rabbit IgG diluted 1:4000 and color development using 4-CN substrate until sufficient color develops.

PBS, pH 7.2, 0.01% Sodium Azide

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.