Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Yeast (tested or 100% immunogen sequence identity)
Delipidated and defibrinated
ELISA (1:1000 - 1:3000)
Specificity and Use
Carboxypeptidase Y [Baker's Yeast].
Carboxypeptidase Y [Baker's Yeast]. Cross reactivity against Carboxypeptidase Y from other tissues and species may occur but have not been specifically determined
Suitable for immunoblotting (western or dot blot) and ELISA. This product has been assayed against 1.0 ug of Carboxypeptidase Y [Baker's Yeast] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884 and ABTS as a substrate for 30 minutes at room temperature.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Anti-Carboxypeptidase Antibody - Western Blot. Both the antiserum and IgG fractions of anti-Carboxypeptidase Y (Bakers Yeast) are shown to detect under reducing conditions of SDS-PAGE the 61000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (LS-C60884) diluted 1:4000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.
Requested From: United States
Date Requested: 2/22/2017