Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Yeast Carboxypeptidase Y
Western blot, Immunoprecipitation, ELISA
Yeast Carboxypeptidase Y
Yeast (tested or 100% immunogen sequence identity)
Ion exchange chromatography
Western blot (1:2500)
ELISA (1:10000 - 1:42000)
Specificity and Use
Carboxypeptidase Y (yeast).
Immunoelectrophoretic assay gives a single precipitin arc against anti-rabbit serum, purified carboxypeptidase Y and partially purified carboxypeptidase Y (yeast). Although not tested, the antibody should exhibit cross-reactivity with other tissues and species.
Suitable for use in Western Blot (Western or Dot Blot), ELISA, Immunoprecipitation and Conjugation. ELISA: 1:10000-1:42000. Western Blot: 1:2500. Both the antiserum and IgG fractions of anti-carboxypeptidase Y (Baker's yeast) are shown to detect under reducing conditions of SDS-PAGE the 61kD enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5000 dilution of the primary antibody is used followed by detection using HRP goat-a-rabbit IgG diluted 1:4000 and color development using 4-CN substrate until sufficient color develops.