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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CAPN5 / Calpain 5 Antibody (clone 3C10) LS‑C196784
Calpain 5 antibody LS-C196784 is an unconjugated mouse monoclonal antibody to human Calpain 5 (CAPN5). Validated for ELISA and WB.
Catalog
Size
Price
LS-C196784-200
200 µl
Unavailable

Popular CAPN5 / Calpain 5 Products

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Species: Human, Mouse, Rat
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Antibody
Species: Human, Mouse
Applications: IHC, Western blot
Immunofluorescent analysis of Calpain 5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Species: Human, Mouse, Rat
Applications: ICC, Immunofluorescence, Western blot
Antibody
Species: Mouse
Applications: IHC, Western blot

Product Description

Calpain 5 antibody LS-C196784 is an unconjugated mouse monoclonal antibody to human Calpain 5 (CAPN5). Validated for ELISA and WB.
About CAPN5 / Calpain 5
Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. O15484 NM_004055 NP_004046.2

CAPN5 Antibody, Calpain-5 Antibody, Calpain htra-3 Antibody, Calpain-like protease 3 Antibody, Htra-3 Antibody, NCL-3 Antibody, Calpain 5 Antibody, NCL3 Antibody, New calpain 3 Antibody


Specifications

Target
Human CAPN5 / Calpain 5
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [3C10]
Conjugations
Unconjugated
Purification
Purified from ascites.
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
CAPN5 (NP_004046, aa523-622 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CAPN5 / Calpain 5
Usage
Western Blot: Cell lysate.
Presentation
Ascites fluid
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

CAPN5 monoclonal antibody (M02A), clone 3C10 Western Blot analysis of CAPN5 expression in HeLa.
CAPN5 monoclonal antibody (M02A), clone 3C10 Western Blot analysis of CAPN5 expression in HeLa.

Western blot

CAPN5 monoclonal antibody (M02A), clone 3C10 Western Blot analysis of CAPN5 expression in HeLa.
CAPN5 monoclonal antibody (M02A), clone 3C10 Western Blot analysis of CAPN5 expression in HeLa.

Western blot

CAPN5 monoclonal antibody (M02A), clone 3C10 Western Blot analysis of CAPN5 expression in HeLa.
CAPN5 monoclonal antibody (M02A), clone 3C10 Western Blot analysis of CAPN5 expression in HeLa.

Western blot

CAPN5 monoclonal antibody (M02A), clone 3C10 Western Blot analysis of CAPN5 expression in HeLa.
CAPN5 monoclonal antibody (M02A), clone 3C10 Western Blot analysis of CAPN5 expression in HeLa.


Requested From: 
Date Requested: 6/24/2018

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