Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG1) to Human CAPN1 / Calpain 1
Human, Rat, Bovine
ICC, Western blot, Flow Cytometry
Human CAPN1 / Calpain 1
Human, Rat, Bovine (tested or 100% immunogen sequence identity)
Specificity and Use
CAPN1 / Calpain 1 antibody was raised against purified bovine skeletal muscle 80 kD subunit of M Calpain. Epitope: This antibody recognizes an epitope between amino acids 502-699 (domain III / IV) of human M Calpain.
Detects the 80kD subunit of M Calpain in human platelets and erythrocytes; bovine platelets, heart and skeletal muscle; rat myoblasts, kidney, liver and spleen; and cultured porcine cells. This antibody does not cross-react with Mu Calpain, N Calpain, calmodulin or calpastatin. Species cross-reactivity: Cross-reacts with Human, Rat, bovine and porcine. Not yet tested in other species.
Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Detects human Calpain M.
0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.