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Anti-CAMKK1 Antibody (clone 1F6) IHC-plus™ LS-B5477

Note: This antibody replaces LS-C133822

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CAMKK1 Antibodies

Anti-CAMKK1 Antibody (aa487-505) LS-C50418
Rabbit Polyclonal to Human CAMKK1
Human, Rat
IHC
Unconjugated
Immunohistochemistry Image
Anti-CAMKK1 Antibody (aa1-132) LS-C77620
Sheep Polyclonal (IgG) to Human CAMKK1
Human, Rat
Western blot
Unconjugated
Anti-CAMKK1 Antibody (clone 1F6) IHC-plus™ LS-B5477
Mouse Monoclonal [clone 1F6] (IgG1,k) to Human CAMKK1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1F6] (IgG1,k) to Human CAMKK1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human CAMKK1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [1F6]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA

Specificity and Use

CAMKK1 antibody was raised against cAMKK1 (AAH31647, 1 a.a. ~ 521 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human CaMKK
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2. Sourced in Ascites.
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About CAMKK1

Q8N5S9 NM_032294 NP_115670.1

CAMKK1 Antibody, CaM-kinase IV kinase Antibody, CaM-kinase kinase 1 Antibody, CaM-kinase kinase alpha Antibody, CAMKK alpha protein Antibody, CAMKKA Antibody, CaM-KK 1 Antibody, CaMKK Antibody, CaM-KK alpha Antibody, CaMKK 1 Antibody, CaMKK alpha Antibody, CAMKKalpha Antibody

Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death.

Immunohistochemistry

Immunohistochemistry
Anti-CAMKK1 / CaMKK antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5477 concentration 5 ug/ml.

Western blot

Western blot
Western blot of CAMKK1 expression in Jurkat cells by CAMKK1 monoclonal antibody, clone 1F6.

Western blot

Western blot
Western blot of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody, clone 1F6.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged CAMKK1 is approximately 1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of CAMKK1 over-expressed 293 cell line, cotransfected with CAMKK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMKK1 monoclonal antibody clone 1F6. GAPDH (36.1 kD) used as control.
 

Requested From: United States
Date Requested: 12/2/2016

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