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Anti-CAMK2 / CAMKII Antibody IHC-plus™ LS-B3743

Note: This antibody replaces LS-C63160

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CAMK2 / CAMKII Antibodies

Anti-CAMK2 / CAMKII Antibody (aa509-558) LS-C119025
Rabbit Polyclonal (IgG) to Human CAMK2 / CAMKII
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
Western blot Image
Anti-CAMK2 / CAMKII Antibody (phospho-Thr286/Thr287) LS-C35252
Mouse Monoclonal (IgG1) to Rat CAMK2 / CAMKII
Rat
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-CAMK2 / CAMKII Antibody (phospho-Thr286) IHC-plus™ LS-B2637
Rabbit Polyclonal to Rat CAMK2 / CAMKII
Rat, Human, Mouse, Xenopus
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Rat CAMK2 / CAMKII
Rat, Human, Monkey, Mouse, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated

Details

Rat CAMK2 / CAMKII
Rabbit
Rat, Human, Monkey, Mouse, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)

Specificity and Use

CAMK2 / CAMKII antibody was raised against a 14 residue synthetic peptide CTRFTDEYQLFEEL based on the rat multifunctional calmodulin-dependent kinase II delta isoform (residues 7-20) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%); Orangutan (93%).
Detects proteins of 50-60 kD, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II alpha subunits expressed in sf9 insect cells are also detected. As the sequences of the rat alpha, delta, and gamma isoforms are conserved over this amino terminus region, this antibody is expected to recognize the delta, gamma and alpha isoforms. This antibody is not expected to cross-react with the beta isoform. Proteins of unknown identity, ~40, 45 and 90 kD, may be detected on immunoblot analysis with some lysates.
Immunohistochemistry: LS-B3743 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3743 was determined to be 10 ug/ml.

Packaging

BBS, pH 8.2 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
Store at -20°C.
For research use only.

About CAMK2 / CAMKII


Immunohistochemistry

Immunohistochemistry
Anti-CAMK2 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3743 concentration 10 ug/ml.

Western blot

Western blot
 

Requested From: United States
Date Requested: 12/9/2016

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