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Anti-CALR / Calreticulin Antibody (clone 1G6A7) IHC-plus™ LS-B3473

Note: This antibody replaces LS-C41528, LS-C171397

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CALR / Calreticulin Antibodies

Anti-CALR / Calreticulin Antibody IHC-plus™ LS-B9387
Rabbit Polyclonal to Human CALR / Calreticulin
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Hamster, Pig, Rabbit, Sheep, Chicken
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-CALR / Calreticulin Antibody (aa1-417, APC) LS-C253324
Rabbit Polyclonal (IgG) to Human CALR / Calreticulin
Human, Mouse
IHC, Western blot, Flow Cytometry, ELISA
APC Conjugated
Anti-CALR / Calreticulin Antibody (FITC) LS-C253327
Rabbit Polyclonal (IgG) to Human CALR / Calreticulin
Human, Mouse
IHC, Western blot, Flow Cytometry, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 1G6A7] (IgG2a) to Human CALR / Calreticulin
Human, Monkey
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human CALR / Calreticulin
Mouse
Human, Monkey (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [1G6A7]
Unconjugated
Ascites
Unmodified

Applications

  • IHC - Paraffin (1:200)
  • Immunofluorescence (1:200 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000)

Specificity and Use

CALR / Calreticulin antibody was raised against synthetic peptide from human CALR / Calreticulin. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset (100%); Gorilla (92%); Bat, Horse (85%).
Synthetic peptide corresponding to aa (EEEDVPGQAKDELC)of human Calreticulin
Immunohistochemistry: LS-B3473 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3473 was determined to be 1:200.

Packaging

Ascites.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About CALR / Calreticulin

P27797 NM_004343 NP_004334.1

CALR Antibody, Calregulin Antibody, CC1qR Antibody, CRP55 Antibody, CRT Antibody, CRTC Antibody, ERp60 Antibody, Grp60 Antibody, RO Antibody, SSA Antibody, Calreticulin Antibody, HACBP Antibody

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors.

Immunohistochemistry

Immunohistochemistry
Anti-Calreticulin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3473 dilution 1:200.

Immunohistochemistry

Immunohistochemistry
Anti-Calreticulin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3473 dilution 1:200.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of SKBR-3 (left) and A549 (right) cells using Calreticulin mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescence of 3T3-L1 cells using Calreticulin mouse monoclonal antibody(green). Blue: DRAQ5 fluorescent DNA dye.

Western blot

Western blot
Western blot using Calreticulin mouse monoclonal antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY Calreticulin cDNA (2).

Western blot

Western blot
Western blot using Calreticulin mouse monoclonal antibody against HeLa (1), A549 (2), NTERA2 (3) and MCF-7 (4) cell lysate.

Requested From: United States
Date Requested: 12/9/2016

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