Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone J4D8] (IgG2a,k) to Human Calmodulin
IHC - Paraffin, Western blot, ELISA
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [J4D8]
Protein G purified
IHC - Paraffin (10 µg/ml)
Western blot (1:500 - 1:1000)
Specificity and Use
Calmodulin antibody was raised against recombinant human Calmodulin (1-149aa) purified from E. coli.
Immunohistochemistry: LS-B2955 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2955 was determined to be 10 ug/ml.
PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
Anti-Calmodulin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2955 concentration 10 ug/ml.
Anti-Calmodulin antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2955 concentration 10 ug/ml.
Cell lysates of NIH3T3 (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Calmodulin (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Requested From: United States
Date Requested: 2/27/2017