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Catalog Number Size Price
LS-C168239-400 400 µl $393 
CA2 / Carbonic Anhydrase II Antibody - Fluorescent confocal image of HeLa cell stained with CA2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly.
CA2 / Carbonic Anhydrase II Antibody - Western blot of lysates from MCF-7 cell line and mouse kidney tissue lysate (from left to right), using CA2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
CA2 / Carbonic Anhydrase II Antibody - CA2 Antibody western blot of MCF-7 cell line and mouse kidney tissue lysates (35 ug/lane). The CA2 antibody detected the CA2 protein (arrow).
CA2 / Carbonic Anhydrase II Antibody - Fluorescent confocal image of HeLa cell stained with CA2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly.
CA2 / Carbonic Anhydrase II Antibody - Western blot of lysates from MCF-7 cell line and mouse kidney tissue lysate (from left to right), using CA2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
CA2 / Carbonic Anhydrase II Antibody - CA2 Antibody western blot of MCF-7 cell line and mouse kidney tissue lysates (35 ug/lane). The CA2 antibody detected the CA2 protein (arrow).
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Polyclonal Rabbit anti‑Human CA2 / Carbonic Anhydrase II Antibody (aa54‑83, IF, WB) LS‑C168239

Polyclonal Rabbit anti‑Human CA2 / Carbonic Anhydrase II Antibody (aa54‑83, IF, WB) LS‑C168239

Antibody:
CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (aa54-83) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C168239-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (aa54-83) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Carbonic Anhydrase II antibody LS-C168239 is an unconjugated rabbit polyclonal antibody to Carbonic Anhydrase II (CA2) (aa54-83) from human. It is reactive with human and mouse. Validated for IF and WB.
Target
Human CA2 / Carbonic Anhydrase II
Synonyms
CA2 | CA-II | CAII | CAC | Car2 | Carbonate dehydratase II | Carbonic anhydrase C | Carbonic anhydrase II | Carbonic dehydratase | Carbonic anhydrase 2 | Carbonic anhydrase B
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa54-83
Specificity
This CA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CA2.
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CA2 / Carbonic Anhydrase II
P00918 NM_000067 NP_000058.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/25/2024