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Anti-CA2 / Carbonic Anhydrase II Antibody (aa54-83) LS-C168239

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CA2 / Carbonic Anhydrase II Antibodies

Anti-CA2 / Carbonic Anhydrase II Antibody IHC-plus™ LS-B692
Rabbit Polyclonal to Human CA2 / Carbonic Anhydrase II
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-CA2 / Carbonic Anhydrase II Antibody IHC-plus™ LS-B1439
Rabbit Polyclonal to Bovine CA2 / Carbonic Anhydrase II
Bovine, Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human CA2 / Carbonic Anhydrase II
Human, Mouse
Immunofluorescence, Western blot
Unconjugated

Details

Human CA2 / Carbonic Anhydrase II
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)

Specificity and Use

CA2 / Carbonic Anhydrase II antibody was raised against kLH-conjugated synthetic peptide between amino acids 54-83 from N-terminal region of human CA2.
LS-E8847 - Lyophilized - 100 µg - $145.00
aa54-83
Human Carbonic Anhydrase II / CA2

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About CA2 / Carbonic Anhydrase II

P00918 NM_000067 NP_000058.1

CA2 Antibody, CA-II Antibody, CAII Antibody, CAC Antibody, Car2 Antibody, Carbonate dehydratase II Antibody, Carbonic anhydrase C Antibody, Carbonic anhydrase II Antibody, Carbonic dehydratase Antibody, Carbonic anhydrase 2 Antibody, Carbonic anhydrase B Antibody

Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cell stained with CA2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly.

Western blot

Western blot
Western blot of lysates from MCF-7 cell line and mouse kidney tissue lysate (from left to right), using CA2 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot

Western blot
CA2 Antibody western blot of MCF-7 cell line and mouse kidney tissue lysates (35 ug/lane). The CA2 antibody detected the CA2 protein (arrow).
 

Requested From: United States
Date Requested: 12/8/2016

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