Target
Human C4.4A / LYPD3
Synonyms
LYPD3 | C4.4A | MIG-C4 | LY6/PLAUR domain containing 3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Purified, NS0-derived, recombinant human C4.4A (rhC4.4A; aa 31-286; Accession# O95274).
Specificity
Recognizes human C4.4A. In direct ELISA, shows ~15% cross-reactivity with rmC4.4A.
Applications
- Western blot (1 µg/ml)
- ELISA
Usage
Suitable for use in ELISA and Western Blot. ELISA (direct): 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human C4.4A. The detection limit for rhC4.4A is approximately 0.2 ng/well. Western Blot: 1.0 ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1 ug/ml sheep anti-hC4.4A.
Presentation
Lyophilized from PBS, 5% Trehalose
Reconstitution
Reconstitute in sterile PBS.
Storage
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee