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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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C‑TAK1 / MARK3 Antibody LS‑C140488
MARK3 antibody LS-C140488 is an unconjugated sheep polyclonal antibody to human MARK3 (C-TAK1). Validated for IP and WB.
Catalog
Size
Price
LS-C140488-250
250 µg
Unavailable

Popular C-TAK1 / MARK3 Products

Anti-MARK3 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5226 concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Anti-MARK3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7053 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded bladder cancer tissues using MARK3 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry, ELISA
Immunohistochemical analysis of MARK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Western blot of C-TAK1 antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

MARK3 antibody LS-C140488 is an unconjugated sheep polyclonal antibody to human MARK3 (C-TAK1). Validated for IP and WB.
About C-TAK1 / MARK3
MARK3, a MARK type protein kinase, was originally identified as a marker induced with DNA damaging agents. The protein contains a N-terminal kinase domain. It has been shown to bind and phosphorylate CDC25C and to regulate Ras signaling by phosphorylating the MAPK scaffold, KSR1. At least three mRNA transcripts have been reported -- 3.0-, 3.1-, and 3.8-kb. Loss of MARK3 has been associated with carcinogenesis in the pancreas. P27448 NM_002376 NP_002367.5

MARK3 Antibody, C-TAK1 Antibody, ELKL motif kinase 2 Antibody, EMK2 Antibody, Etk-1 Antibody, KP78 Antibody, PAR1A Antibody, Ser/Thr protein kinase PAR-1 Antibody, Par-1a Antibody, CTAK1 Antibody, EMK-2 Antibody, Protein kinase STK10 Antibody


Specifications

Target
Human C-TAK1 / MARK3
Host
Sheep
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
Immunogen
C-TAK1 / MARK3 antibody was raised against recombinant, full-length His-tagged human C-TAK1 produced in E. coli.
Specificity
Human MARK3
Usage
Antibody detects c-tak1 by Western blot analysis at 1 to 5 ug/ml. Detects c-tak1 in RKO and HCT116 cell lysates. Optimal concentration should be evaluated by serial dilutions.
Presentation
PBS, 0.08% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of c-tak1 antibody (LS-C140488) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3).
Western blot of c-tak1 antibody (LS-C140488) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3).

Western blot

Western blot of c-tak1 antibody (LS-C140488) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3).
Western blot of c-tak1 antibody (LS-C140488) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3).

Western blot

Western blot of c-tak1 antibody (LS-C140488) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3).
Western blot of c-tak1 antibody (LS-C140488) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3).

Western blot

Western blot of c-tak1 antibody (LS-C140488) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3).
Western blot of c-tak1 antibody (LS-C140488) on His-tagged c-tak1 (1), RKO cell lysate (2) and HCT116 cell lysate (3).


Requested From: 
Date Requested: 6/22/2018

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