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Anti-c-Src Kinase / CSK Antibody (clone 3A3) LS-C133138

Catalog Size Price
LS-C133138-100 100 µg Unavailable

Most Popular c-Src Kinase / CSK Antibodies

Anti-c-Src Kinase / CSK Antibody (Val399) LS-C63922
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Anti-c-Src Kinase / CSK Antibody (clone 5F3) LS-C169267
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Immunofluorescence Image
Anti-c-Src Kinase / CSK Antibody (aa376-404, Azide-free, HRP) LS-C255517
Rabbit Polyclonal (IgG) to Human c-Src Kinase / CSK
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HRP Conjugated, Azide-free
Anti-c-Src Kinase / CSK Antibody (aa375-425) IHC-plus™ LS-B14203
Rabbit Polyclonal to Human c-Src Kinase / CSK
Human, Mouse, Rat, Bovine, Dog, Horse, Chicken
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Immunohistochemistry - Paraffin Image
Anti-c-Src Kinase / CSK Antibody (aa400-450) IHC-plus™ LS-B14204
Rabbit Polyclonal to Human c-Src Kinase / CSK
Human, Monkey, Mouse, Dog, Pig
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed
Mouse Monoclonal [clone 3A3] (IgG1,k) to Human c-Src Kinase / CSK
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human c-Src Kinase / CSK
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [3A3]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • Western blot
  • Immunoprecipitation
  • ELISA

  • IHC - Paraffin

Specificity and Use

CSK (NP_004374, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human CSK
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About c-Src Kinase / CSK

P41240 NM_004383 NP_004374.1

CSK Antibody, C-src tyrosine kinase Antibody, C-Src kinase Antibody, Protein-tyrosine kinase CYL Antibody, Tyrosine-protein kinase CSK Antibody, C-SRC Antibody, Cytoplasmic tyrosine kinase Antibody

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation.

Western blot

Western blot
Western blot of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.

Western blot

Western blot
CSK monoclonal antibody (M01), clone 3A3. Western blot of CSK expression in HeLa NE.

Western blot

Western blot
CSK monoclonal antibody (M01), clone 3A3 Western blot of CSK expression in HL-60.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of CSK transfected lysate using anti-CSK monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged CSK is approximately 0.1 ng/ml as a capture antibody.

RNA interference

RNA interference
Western blot of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody clone 3A3. GAPDH ( 36.1 kD ) used as specificity and lo.

Requested From: 
Date Requested: 8/17/2017

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