Human c-Met

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

A phosphopeptide containing human HGF R/c-MET Y1234/1235 sites.

pTyr1234/Tyr1235

Detects endogenous human HGF R/c-MET phosphorylated at Y1234/Y1235.

• Western blot
• Flow Cytometry

Western Blot: An antibody concentration of 0.5 ug/ml is recommended. Dilute this antibody to 25 ug/ml and add 10 ul of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200 L. Following a 30 minute incubation, cells should be washed in an isotonic phosphate buffer (supplemented with 0.5% BSA) prior to the addition of a secondary developing reagent. The binding of unlabeled polyclonal antibodies may be visualized by adding 10 ul of a 25 ug/ml solution of a secondary developing reagent such as anti-rabbit IgG conjugated to a fluorochrome. Cells should be washed for a final time prior to flow cytometric analysis.

Lyophilized

Reconstitute in deionized water

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08581 NCBI: NM_000245 NP_000236.2