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Anti-c-Met Antibody (phospho-Tyr1234/Tyr1235) LS-C150281

Ordering

Wt. Vol. Conc. Price
50 µg - 0.2 mg/ml $345
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular c-Met Antibodies

Anti-c-Met Antibody (Cytoplasmic Domain) IHC-plus™ LS-B2812
Mouse Monoclonal (IgG2a) to Human c-Met
Human
IHC - Paraffin, IHC - Frozen
Unconjugated
Immunohistochemistry Image
Anti-c-Met Antibody (phospho-Tyr1234/Tyr1235) LS-C150281
Rabbit Polyclonal (IgG) to Human c-Met
Human, Mouse
IHC, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human c-Met
Human, Mouse
IHC, Western blot, Flow Cytometry
Unconjugated

Details

Human c-Met
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC (5 - 15 µg/ml)
  • Western blot (0.5 µg/ml)
  • Flow Cytometry

Specificity and Use

c-Met antibody was raised against phosphopeptide containing human HGF R/c-MET Y1234/1235 sites.
pTyr1234/Tyr1235
Detects human and mouse HGF R/c-Met when phosphorylated at Y1234/Y1235 in Western blots.
Western Blot, 0.5 µg/ml. Immunohistochemistry, 5-15 µg/ml, Intracellular Staining by Flow Cytometry, 2.5 µg/106 cells.

Packaging

Lyophilized from 0.2 µm filtered PBS, pH 7.4, 5% trehalose
Sterile PBS. Resuspend to 250 ul
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About c-Met

P08581 NM_000245 NP_000236.2

MET Antibody, AUTS9 Antibody, C-Met Antibody, HGF/SF receptor Antibody, HGFR Antibody, Met kinase Antibody, Proto-oncogene c-Met Antibody, Scatter factor receptor Antibody, SF receptor Antibody, HGF receptor Antibody, RCCP2 Antibody, Tyrosine-protein kinase Met Antibody

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules.

Immunohistochemistry

Immunohistochemistry
HGFR/c-MET in Mouse Embryo. HGFR/c-MET was detected in immersion fixed frozen sections of mouse embryo (13 d. p. c.) using Human Phospho-HGF R/c-MET (Y1234/Y1235) Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Rabbit HRP-DAB (brown) and counterstained with hematoxylin (blue).

Western blot

Western blot
Detection of Human Phospho HGFR/c-MET (Y1234/Y1235) by Western Blot. Western blot shows Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody immunoprecipitate of MDA-MB-468 human breast cancer cell line untreated (-) or treated (+) with 100 uM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 ug/ml of Human Phospho-HGF R/c-MET (Y1234/Y1235) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for Phospho HGFR/c-MET (Y1234/Y1235) at approximately 145 kD (as indicated). This experiment was conducted under reducing conditions.

Flow Cytometry

Flow Cytometry
Detection of HGF R/c-MET in pervanadate-treated MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was unstimulated (light orange open histogram) or treated with 100 uM pervanadate for 10 minutes (dark orange filled histogram), then stained with Human Phospho-HGF R/c-MET (Y1234/Y1235) Antigen Affinity-purified Polyclonal Antibody, or control antibody, followed by Phycoerythrinconjugated Anti-Rabbit IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
 

Requested From: United States
Date Requested: 12/8/2016

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