Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
MET Antibody, AUTS9 Antibody, C-Met Antibody, HGF/SF receptor Antibody, HGFR Antibody, Met kinase Antibody, Proto-oncogene c-Met Antibody, Scatter factor receptor Antibody, SF receptor Antibody, HGF receptor Antibody, RCCP2 Antibody, Tyrosine-protein kinase Met Antibody
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules.
HGFR/c-MET in Mouse Embryo. HGFR/c-MET was detected in immersion fixed frozen sections of mouse embryo (13 d. p. c.) using Human Phospho-HGF R/c-MET (Y1234/Y1235) Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Rabbit HRP-DAB (brown) and counterstained with hematoxylin (blue).
Detection of Human Phospho HGFR/c-MET (Y1234/Y1235) by Western Blot. Western blot shows Human HGF R/c-MET Antigen Affinity-purified Polyclonal Antibody immunoprecipitate of MDA-MB-468 human breast cancer cell line untreated (-) or treated (+) with 100 uM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 ug/ml of Human Phospho-HGF R/c-MET (Y1234/Y1235) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for Phospho HGFR/c-MET (Y1234/Y1235) at approximately 145 kD (as indicated). This experiment was conducted under reducing conditions.
Detection of HGF R/c-MET in pervanadate-treated MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was unstimulated (light orange open histogram) or treated with 100 uM pervanadate for 10 minutes (dark orange filled histogram), then stained with Human Phospho-HGF R/c-MET (Y1234/Y1235) Antigen Affinity-purified Polyclonal Antibody, or control antibody, followed by Phycoerythrinconjugated Anti-Rabbit IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.