(tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
- Western blot (1:1000)
c-Met antibody was raised against synthetic phosphopeptide derived from the region of human c-Met that contains tyrosines 1230, 1234 and 1235. The sequence is conserved in mouse and rat.
Recognizes human and mouse c-Met. c-Met has not been tested. The phosphospecific antibody that has been generated does not distinguish between the dually (pYpY1234/1235) and triply (pYpYpY1230/1234/1235) phosphorylated forms of c-Met, both of which are likely to represent activated forms of this receptor. Species sequence homology: rat 100%.
Suitable for use in Western Blot and ELISA. Western Blot: 1:1000. Positive control: 293T human kidney cells transiently transfected with human Met cDNA, stimulated with HGF; Mouse myeloma (SP-1) cells stimulated with HGF; A431 cells stimulated with EGF.
1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.3, 0.05% Sodium Azide, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.