C-Met antibody LS-B2812 is an unconjugated mouse monoclonal antibody to human c-Met. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen (1:50)
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c-Met antibody was raised against synthetic peptide derived from cytoplasmic domain of human c-Met protein.
Immunohistochemistry: LS-B2812 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2812 was determined to be 10 ug/ml. Positive control: Breast cancer.
PBS, pH 7.4, 0.1% Sodium Azide, 1% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.