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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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c‑Met Antibody (C‑Terminus) LS‑C16510
C-Met antibody LS-C16510 is an unconjugated rabbit polyclonal antibody to human c-Met. Validated for ELISA, IHC and WB.
Antibody
Catalog
Size
Price
LS-C16510-100
100 µg
$605

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Product Description

C-Met antibody LS-C16510 is an unconjugated rabbit polyclonal antibody to human c-Met. Validated for ELISA, IHC and WB.

Specifications

Target
Human c-Met
Synonyms
MET, AUTS9, C-Met, HGF/SF receptor, HGFR, Met kinase, Proto-oncogene c-Met, Scatter factor receptor, SF receptor, HGF receptor, RCCP2, Tyrosine-protein kinase Met
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1 - 2 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
c-Met antibody was raised against synthetic peptide corresponding to 13aa located within last 50aa derived from the C-terminus of the human c-Met protein.
Epitope
C-Terminus
Specificity
Recognizes the 145kD human c-Met protein. Does not cross-react with related proteins.
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Western Blot: 1-2 ug/ml. Immunohistochemistry (paraffin): 1-2 ug/ml. Best results obtained by subjecting tissue to heat-induced epitope retrieval (HIER). Positive control: WB: A431 cell extracts.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only.
About c-Met
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. P08581 NM_000245 NP_000236.2


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Requested From: United States
Date Requested: 10/21/2018

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