Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone X1] (IgG2b,k) to Human c-Kit / CD117
IHC - Paraffin, Western blot, ELISA
Rat Monoclonal (IgG2b) to Mouse c-Kit / CD117
Mouse c-Kit / CD117
Mouse (tested or 100% immunogen sequence identity)
Protein G purified
Flow Cytometry (1:50 - 1:200)
Specificity and Use
c-Kit / CD117 antibody was raised against iL-3 dependent mast cells.
Recognizes mouse CD117, a 150kD cell surface molecule that is otherwise known as the Kit, or c-kit, receptor. Recognizes an epitope within the extracellular region of CD117. In mice, CD117 is predominantly expressed on multipotent hematopoietic stem cells from the adult bone marrow and fetal liver.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
PBS, pH 7.4, 0.09% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL.