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c‑Kit / CD117 Antibody (clone YB5.B8, PE) LS‑C106342
CD117 antibody LS-C106342 is a PE-conjugated mouse monoclonal antibody to human CD117 (c-Kit). Validated for Flow.
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Review This ProductPopular c-Kit / CD117 Products
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human, Rat
Applications:
IHC, Immunofluorescence, Flow Cytometry
Species:
Mouse
Applications:
IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Species:
Human, Dog
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Product Description
CD117 antibody LS-C106342 is a PE-conjugated mouse monoclonal antibody to human CD117 (c-Kit). Validated for Flow.
About c-Kit / CD117
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL.
P10721
NM_000222
NP_000213.1
KIT Antibody, C-Kit Antibody, CD117 Antibody, PBT Antibody, Piebald trait Antibody, Piebald trait protein Antibody, Proto-oncogene c-Kit Antibody, SCFR Antibody, Soluble KIT variant 1 Antibody, Tyrosine-protein kinase Kit Antibody, p145 c-kit Antibody, CD117 antigen Antibody
Specifications
Target
Human c-Kit / CD117
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
[YB5.B8]
Conjugations
Phycoerythrin
Purification
Affinity purified
Modifications
Unmodified
Applications
- Flow Cytometry
Immunogen
c-Kit / CD117 antibody was raised against human KIT
Usage
The YB5. B8 antibody has been tested by flow cytometric analysis of the TF-1 cell line, a human erythroleukemia cell line. This can be used at 5 ul (0.25 ug)/per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide, 0.2% BSA
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Flow Cytometry
Surface staining of TF-1 cells with anti-human CD117 (YB5. B8) PE. Autofluorescence is indicated by open histogram. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow Cytometry
Surface staining of TF-1 cells with anti-human CD117 (YB5. B8) PE. Autofluorescence is indicated by open histogram. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow Cytometry
Surface staining of TF-1 cells with anti-human CD117 (YB5. B8) PE. Autofluorescence is indicated by open histogram. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Flow Cytometry
Surface staining of TF-1 cells with anti-human CD117 (YB5. B8) PE. Autofluorescence is indicated by open histogram. Total viable cells were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/20/2018
Date Requested: 6/20/2018
