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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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c‑Kit / CD117 Antibody (clone 4F7) IHC‑plus™ LS‑B6059
Note: This antibody replaces LS-C133900
CD117 antibody LS-B6059 is an unconjugated mouse monoclonal antibody to human CD117 (c-Kit). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6059-50
50 µg (0.5 mg/ml)
Unavailable

Popular c-Kit / CD117 Products

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Immunofluorescence staining of rat skin (epidermis). c-KIT detection was done using LS-C78828 (red color). DAPI was used to counterstain cell nuclei (blue color). Working dilution: 1:100-1:300.
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Antibody
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Product Description

CD117 antibody LS-B6059 is an unconjugated mouse monoclonal antibody to human CD117 (c-Kit). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About c-Kit / CD117
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. P10721 NM_000222 NP_000213.1

KIT Antibody, C-Kit Antibody, CD117 Antibody, PBT Antibody, Piebald trait Antibody, Piebald trait protein Antibody, Proto-oncogene c-Kit Antibody, SCFR Antibody, Soluble KIT variant 1 Antibody, Tyrosine-protein kinase Kit Antibody, p145 c-kit Antibody, CD117 antigen Antibody


Specifications

Target
Human c-Kit / CD117
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [4F7]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
c-Kit / CD117 antibody was raised against kIT (NP_000213, 41 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human c-Kit
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates and the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6059 concentration 2.5 ug/ml.
Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6059 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6059 concentration 2.5 ug/ml.
Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6059 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6059 concentration 2.5 ug/ml.
Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6059 concentration 2.5 ug/ml.

Immunohistochemistry

Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6059 concentration 2.5 ug/ml.
Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6059 concentration 2.5 ug/ml.


Requested From: 
Date Requested: 6/18/2018

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