Target
Human c-CBL
Synonyms
CBL | C-CBL | Oncogene CBL2 | RING finger protein 55 | Proto-oncogene c-Cbl | RNF55 | CBL2 | FRA11B | NSLL
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein G purified
Immunogen
GST fusion protein corresponding to residues 878-906 of human Cbl. This sequence is identical in mouse.
Specificity
Specific for Cbl. Species cross-reactivity: mouse and rat.
Applications
- ICC (5 - 10 µg/ml)
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
Usage
WB: Detects Cbl (120kD) in RIPA lysates from human Jurkat and mouse 3T3 cells. 3T3 cell lysate was resolved by electro-phoresis, transferred to nitrocellulose and probed with anti-Cbl (1 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. ICC: A previous lot showed positive immunostaining for Cbl in 3T3 cells fixed with ice cold methanol/ethanol [1:1] for 1 minute. IP: 2ug immunoprecipitated Cbl from 1ug of a mouse 3T3 cell RIPA lysate.
Presentation
PBS, 0.05% Sodium Azide, 20% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee