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Catalog Number Size Price
LS-C60129-100 100 µg (1 mg/ml) $567 
C/EBP Delta / CEBPD Antibody - Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.
C/EBP Delta / CEBPD Antibody - Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kD, ~35 kD.
C/EBP Delta / CEBPD Antibody - Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.
C/EBP Delta / CEBPD Antibody - Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: human C/EBPbeta Lane 2: Mouse C/EBPdelta Lane 3: untransfected Lane 4: human C/EBPdelta Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of overexpressed protein in human and mouse C/EBP delta. Predicted/Observed size: 28.8 kD, ~35 kD.
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Polyclonal Rabbit anti‑Mouse C/EBP Delta / CEBPD Antibody (IHC, WB) LS‑C60129

Polyclonal Rabbit anti‑Mouse C/EBP Delta / CEBPD Antibody (IHC, WB) LS‑C60129

Antibody:
C/EBP Delta / CEBPD Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C60129-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
C/EBP Delta / CEBPD Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified

Specifications

Description
CEBPD antibody LS-C60129 is an unconjugated rabbit polyclonal antibody to CEBPD (C / EBP Delta) from mouse. It is reactive with human and mouse. Validated for ELISA, IHC, IP and WB.
Target
Mouse C/EBP Delta / CEBPD
Synonyms
CEBPD | C/EBP-delta | C/EBP delta | CELF | CRP3 | NF-IL6-beta | Nuclear factor NF-IL6-beta
Host
Rabbit
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to the amino terminus of mouse C/EBP.
Specificity
This antibody recognizes endogenous mouse and recombinant human C/EBP delta protein.  A BLAST analysis was used to suggest cross-reactivity with C/EBPd from human sources based on 77% homology with the immunizing sequence. No cross-reactivity is observed with mouse C/EBPa or C/EBPb. Reactivity with C/EBPd from other sources has not been determined.
Applications
  • IHC (1:2000)
  • Western blot (1:1000 - 1:15000)
  • Immunoprecipitation (0.8 - 1.5 µg/ml)
  • ELISA (1:4000 - 1:9000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 35 kD in size corresponding to C/EBPS by western blotting in the appropriate cell lysate or extract. The predicted molecular weight of mouse C/EBPS is 28.8 kD. This antibody is capable of detecting both over-expressed and endogenous C/EBPS.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About C/EBP Delta / CEBPD
P49716 NM_005195 NP_005186.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/20/2024