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Anti-BUB1B / BubR1 Antibody (clone 2G5) IHC-plus™ LS-B4289

Note: This antibody replaces LS-C104869

Ordering

Wt. Vol. Conc. Price
50 µg - 0.48 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BUB1B / BubR1 Antibodies

Anti-BUB1B / BubR1 Antibody (aa1-350, clone 8G1) LS-C2771
Mouse Monoclonal [clone 8G1] (IgG1,k) to Human BUB1B / BubR1
Human, Mouse
Immunofluorescence, Western blot
Unconjugated
Immunofluorescence Image
Anti-BUB1B / BubR1 Antibody (clone 2G5) IHC-plus™ LS-B4289
Mouse Monoclonal [clone 2G5] (IgG,k) to Human BUB1B / BubR1
Human
IHC - Paraffin, Western blot, ELISA, Proximity Ligation Assay, RNA interference
Unconjugated
Immunohistochemistry Image
Anti-BUB1B / BubR1 Antibody IHC-plus™ LS-B4854
Rabbit Polyclonal to Human BUB1B / BubR1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2G5] (IgG,k) to Human BUB1B / BubR1
Human
IHC - Paraffin, Western blot, ELISA, Proximity Ligation Assay, RNA interference
Unconjugated

Details

Human BUB1B / BubR1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG,k Monoclonal [2G5]
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA
  • Proximity Ligation Assay
  • RNA interference

Specificity and Use

BUB1B / BubR1 antibody was raised against bUB1B (AAH18739, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated). Proximity Ligation Assay (Cell).

Packaging

PBS, pH 7.4.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

About BUB1B / BubR1

O60566 NM_001211 NP_001202.4

BUB1B Antibody, Bub1A Antibody, BUBR1 Antibody, HBUBR1 Antibody, Protein SSK1 Antibody, SSK1 Antibody, BUB1beta Antibody, MAD3L Antibody

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE.

Immunohistochemistry

Immunohistochemistry
Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.

Western blot

Western blot
BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.

Western blot

Western blot
Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.

Proximity Ligation Assay

Proximity Ligation Assay
Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

RNA interference

RNA interference
Western blot of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody clone 2G5. GAPDH ( 36.1 kD ) used as specificity an.
 

Requested From: United States
Date Requested: 12/6/2016

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