LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
BUB1B / BubR1 Antibody (clone 2G5) IHC‑plus™ LS‑B4289
Note: This antibody replaces LS-C104869
BubR1 antibody LS-B4289 is an unconjugated mouse monoclonal antibody to human BubR1 (BUB1B). Validated for ELISA, IHC, PLA, RNAi and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B4289-50
50 µg (0.47 mg/ml)
Unavailable

Popular BUB1B / BubR1 Products

Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with LS-C2771. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse
Applications: Immunofluorescence, Western blot
IHC of paraffin-embedded pancreas tissue using anti-BUB1B mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4291 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-BUB1B / BubR1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4854 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

BubR1 antibody LS-B4289 is an unconjugated mouse monoclonal antibody to human BubR1 (BUB1B). Validated for ELISA, IHC, PLA, RNAi and WB. Tested on 20 paraffin-embedded human tissues.
About BUB1B / BubR1
Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. O60566 NM_001211 NP_001202.4

BUB1B Antibody, Bub1A Antibody, BUBR1 Antibody, HBUBR1 Antibody, Protein SSK1 Antibody, SSK1 Antibody, BUB1beta Antibody, MAD3L Antibody


Specifications

Target
Human BUB1B / BubR1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG,k Monoclonal [2G5]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA
  • Proximity Ligation Assay
  • RNA interference
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BUB1B / BubR1 antibody was raised against bUB1B (AAH18739, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Usage
Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated). Proximity Ligation Assay (Cell).
Presentation
PBS, pH 7.4.
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Immunohistochemistry

Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.

Immunohistochemistry

Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.

Western blot

BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.
BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.

Western blot

Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.
Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.

Proximity Ligation Assay

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

RNA interference

Western blot of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody clone 2G5. GAPDH ( 36.1 kD ) used as specificity an.
Western blot of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody clone 2G5. GAPDH ( 36.1 kD ) used as specificity an.

Immunohistochemistry

Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.

Immunohistochemistry

Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.

Western blot

BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.
BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.

Western blot

Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.
Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.

Proximity Ligation Assay

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

RNA interference

Western blot of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody clone 2G5. GAPDH ( 36.1 kD ) used as specificity an.
Western blot of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody clone 2G5. GAPDH ( 36.1 kD ) used as specificity an.

Immunohistochemistry

Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.

Immunohistochemistry

Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.

Western blot

BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.
BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.

Western blot

Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.
Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.

Proximity Ligation Assay

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

RNA interference

Western blot of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody clone 2G5. GAPDH ( 36.1 kD ) used as specificity an.
Western blot of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody clone 2G5. GAPDH ( 36.1 kD ) used as specificity an.

Immunohistochemistry

Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.

Immunohistochemistry

Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.

Western blot

BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.
BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.

Western blot

Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.
Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.

Proximity Ligation Assay

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.
Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

RNA interference

Western blot of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody clone 2G5. GAPDH ( 36.1 kD ) used as specificity an.
Western blot of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody clone 2G5. GAPDH ( 36.1 kD ) used as specificity an.


Requested From: 
Date Requested: 6/21/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number