Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IgG,k Monoclonal [2G5]
Purified from ascites by Protein A
IHC - Paraffin (5 µg/ml)
Proximity Ligation Assay
Specificity and Use
BUB1B / BubR1 antibody was raised against bUB1B (AAH18739, 1 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated). Proximity Ligation Assay (Cell).
PBS, pH 7.4.
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE.
Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.
Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4289 concentration 5 ug/ml.
BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.
Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.
Proximity Ligation Assay
Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.
Western blot of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody clone 2G5. GAPDH ( 36.1 kD ) used as specificity an.