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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BTN1A1 Antibody (clone 3A12) LS‑C115498
BTN1A1 antibody LS-C115498 is an unconjugated mouse monoclonal antibody to human BTN1A1. Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C115498-100
100 µl
Unavailable

Popular BTN1A1 Products

Anti-BTN1A1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.
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Applications: Immunofluorescence, Western blot, Flow Cytometry
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-BTN1A1 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Anti-BTN1A1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.
Species: Human
Applications: Immunofluorescence, Western blot, Flow Cytometry
Western blot analysis of extracts of various cells.
Species: Human, Mouse, Rat
Applications: Western blot

Product Description

BTN1A1 antibody LS-C115498 is an unconjugated mouse monoclonal antibody to human BTN1A1. Validated for Flow, IF and WB.
About BTN1A1
Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families. Q13410 NM_001732 NP_001723.2

BTN1A1 Antibody, BTN Antibody, BT Antibody, Butyrophilin Antibody


Specifications

Target
Human BTN1A1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [3A12]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:200 - 1:500)
  • Flow Cytometry (1:100)
Immunogen
BTN1A1 antibody was raised against full length human recombinant protein of human BTN1A1 (NP_001723) produced in HEK293T cell.
Specificity
Human BTN1A1
Usage
Concentration: 0.56 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Anti-BTN1A1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.
Anti-BTN1A1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.

Flow Cytometry

Flow cytometry of HeLa cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of HeLa cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTN1A1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTN1A1 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-BTN1A1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.
Anti-BTN1A1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.

Flow Cytometry

Flow cytometry of HeLa cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of HeLa cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTN1A1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTN1A1 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-BTN1A1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.
Anti-BTN1A1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.

Flow Cytometry

Flow cytometry of HeLa cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of HeLa cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTN1A1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTN1A1 antibody, and then analyzed by flow cytometry.

Immunofluorescence

Anti-BTN1A1 mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.
Anti-BTN1A1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1.

Flow Cytometry

Flow cytometry of HeLa cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of HeLa cells, using anti-BTN1A1 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTN1A1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTN1A1 antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/17/2018

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