Target
Human BTLA / CD272
Synonyms
BTLA | BTLA1 | B and T lymphocyte associated | CD272 | B and T lymphocyte attenuator | B- and T-lymphocyte attenuator | CD272 antigen
Reactivity
Human, Monkey, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence KEAPTEYASICVRS, from the C-Terminus of protein sequence according to NP_861445.3NP_001078826.1.
Specificity
Human BTLA. This antibody is expected to recognize both reported isoforms (NP_861445.3; NP_001078826.1).
Applications
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western Blot: Approx. 35kD band observed in Mouse Spleen lysates (calculated MW of 32.8kD according to Human NP_861445.3 and of 34.2kD according to Mouse NP_001032808.2). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee