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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BTK Antibody (clone 12H4) LS‑C115132
BTK antibody LS-C115132 is an unconjugated mouse monoclonal antibody to human BTK. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C115132-100
100 µl
Unavailable

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Product Description

BTK antibody LS-C115132 is an unconjugated mouse monoclonal antibody to human BTK. Validated for Flow, IF, IHC and WB.
About BTK
Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. Q06187 NM_000061 NP_000052.1

BTK Antibody, AGMX1 Antibody, AT Antibody, ATK Antibody, XLA Antibody, PSCTK1 Antibody, B-cell progenitor kinase Antibody, BPK Antibody, Bruton tyrosine kinase Antibody, Tyrosine-protein kinase BTK Antibody, IMD1 Antibody


Specifications

Target
Human BTK
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG3 Monoclonal [12H4]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:500)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BTK antibody was raised against full length human recombinant protein of human BTK (NP_000052) produced in HEK293T cell.
Specificity
Human BTK
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-BTK mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-BTK mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-BTK mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-BTK mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Anti-BTK mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.
Anti-BTK mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY BTK (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/24/2018

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