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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BRN2 / POU3F2 Antibody (clone 1H5) LS‑C139417
POU3F2 antibody LS-C139417 is an unconjugated mouse monoclonal antibody to human POU3F2 (BRN2). Validated for ELISA and WB.
Catalog
Size
Price
LS-C139417-100
100 µg
Unavailable

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Applications: IHC, Western blot, ELISA

Product Description

POU3F2 antibody LS-C139417 is an unconjugated mouse monoclonal antibody to human POU3F2 (BRN2). Validated for ELISA and WB.
About BRN2 / POU3F2
Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters. P20265 NM_005604 NP_005595.2

POU3F2 Antibody, Brain-2 Antibody, Brn-2 Antibody, BRN2 Antibody, Oct-7 Antibody, Octamer-binding protein 7 Antibody, OTF7 Antibody, POU class 3 homeobox 2 Antibody, N-Oct3 Antibody, OCT7 Antibody, OTF-7 Antibody, POUF3 Antibody


Specifications

Target
Human BRN2 / POU3F2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal [1H5]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
BRN2 / POU3F2 antibody was raised against pOU3F2 (NP_005595, 1 a.a. ~ 68 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human POU3F2
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU3F2 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU3F2 is approximately 0.3 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU3F2 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU3F2 is approximately 0.3 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU3F2 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU3F2 is approximately 0.3 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged POU3F2 is approximately 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged POU3F2 is approximately 0.3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/22/2018

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