Target
Human BRN2 / POU3F2
Synonyms
POU3F2 | Brain-2 | Brn-2 | BRN2 | Oct-7 | Octamer-binding protein 7 | OTF7 | POU class 3 homeobox 2 | N-Oct3 | OCT7 | OTF-7 | POUF3
Reactivity
Human, Mouse, Rat, Bovine
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-AQSLVQGDYGALQSN, from the internal region (near N-Terminus) of the protein sequence according to NP_005595.2.
Specificity
Human POU3F2.
Applications
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 45kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9kDa according to Human NP_005595.2 and 47.1kDa according to Mouse NP_032925.1). Recommended concentration: 0.1-0.3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee