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Anti-BRIP1 / BACH1 Antibody (clone pp15-IB4) IHC-plus™ LS-B74

Note: This antibody replaces LS-C2623

Ordering

Wt. Vol. Conc. Price
- 100 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BRIP1 / BACH1 Antibodies

Anti-BRIP1 / BACH1 Antibody (C-Terminus) LS-C2622
Rabbit Polyclonal to Human BRIP1 / BACH1
Human
Western blot, Immunoprecipitation
Unconjugated
Western blot Image
Anti-BRIP1 / BACH1 Antibody (clone pp15-IB4) IHC-plus™ LS-B74
Mouse Monoclonal [clone pp15-IB4] (IgG1,k) to Human BRIP1 / BACH1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-BRIP1 / BACH1 Antibody (aa92-104) LS-C121793
Rabbit Polyclonal (IgG) to Human BRIP1 / BACH1
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone pp15-IB4] (IgG1,k) to Human BRIP1 / BACH1
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human BRIP1 / BACH1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [pp15-IB4]
Unconjugated
Unpurified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:10 - 1:500)

Specificity and Use

BRIP1 / BACH1 antibody was raised against a peptide corresponding to the C-terminus of human FANCJ within amino acids 1200-1249 (SwissProt# Q9BX63).
Brip1 /FANCJ.
Immunohistochemistry: LS-B74 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B74 was determined to be 10 ug/ml.

Packaging

Ascites, 0.02% sodium azide
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About BRIP1 / BACH1

Q9BX63 NM_032043 NP_114432.2

BRIP1 Antibody, BRCA1-interacting protein 1 Antibody, Fanconi anemia group J protein Antibody, FANCJ Antibody, Protein FACJ Antibody

DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.

Immunohistochemistry

Immunohistochemistry
Anti-BRIP1 / FANCJ antibody IHC of human spleen, periarteriolar lymphoid sheath. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B74 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/7/2016

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