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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BrdU Antibody LS‑C77272
Antibody
BrdU antibody LS-C77272 is an unconjugated mouse monoclonal antibody to BrdU. Validated for IF.
Catalog
Size
Price
LS-C77272-100
100 µg
Unavailable

Popular BrdU Products

Brdu-incorporated Hut-78 cells stained with purified MoBU-1, followed by anti-mouse IgG FITC
Species: Human
Applications: IHC, Flow Cytometry
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Formalin-fixed, paraffin-embedded, BrdU-labeled mouse small intestine stained with peroxidase-conjugate and DAB chromogen. Note nuclear staining of crypt epithelial cells.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Antibody
Species:
Applications: IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Immunofluorescence microscopy images of paraformaldehyde-fixed, paraffin-embedded pancreas sections stained with antibodies against BrdU (red or pink) and counterstained with DAPI (blue) and imaged with a 40 objective. DAPI stained nuclei (blue) indicate non-dividing cells, immunostained red and pink nuclei indicate actively dividing pancreatic -cells. The antibodies were diluted to 2.7 g/ml. and incubated with tissue sections overnight at 4 degrees. Donkey anti-rabbit secondary antibody was diluted 1:2500.
Species:
Applications: Immunofluorescence

Product Description

BrdU antibody LS-C77272 is an unconjugated mouse monoclonal antibody to BrdU. Validated for IF.
About BrdU
Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. 5-Bromodeoxycytidine is deaminated to form BrdU. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle during which DNA is replicated), substituting for thymidine during DNA replication.

Specifications

Target
BrdU
Host
Mouse
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10)
Immunogen
BrdU antibody was raised against bromodeoxyuridine.
Specificity
Specific for bromodesoxyuridine and does not cross-react with thymidine. It is a valuable tool for detection of S-phase (DNA-synthesizing cells) in cell and tissue culture.
Usage
Suitable for use in Immunofluorescence microscopy. Also suitable for Detection of bromodeoxyuridine incorporation in tissue and cell culture; 3H-thymidine labeling techniques can be avoided. Immunofluorescence microscopy: 1:10; Incubation Time 30 min at RT.
Presentation
Lyophilized from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.
Reconstitution
100 µl Sterile 40-50% glycerol
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/18/2018

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