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Anti-BrdU Antibody (clone IIb5) LS-C134859


Wt. Vol. Conc. Price
- 1000 µl - Unavailable

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100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone IIb5] (IgG1)
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Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [IIb5]


  • IHC - Paraffin
  • IHC - Frozen
  • Immunofluorescence
  • Flow Cytometry

Specificity and Use

BrdU is a thymidine analogue and when offered to proliferating cells it is incorporated into reduplicating DNA. The antibody is specific for DNA in which BrdU has been incorporated. Immunogen: BrdU coupled to BSA. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In Immunocytochemistry the antibody only recognizes BrdU in denatured (single stranded) DNA. The BrdU antibody is 100% cross-reactive with Jodo-Deoxy-Uridine (IrdU). Therefore, IdU instead of BrdU can be used for in vivo studies.
Pulse labeling of dividing cells will allow the immunocytochemical identification of S-phase cells. BrdU incorporating can be analyzed in vitro cell cultures by adding BrdU to the tissue culture medium (10pM final conc.). Exposure periods as brief as 10 min. allow sufficient BrdU Incorporation for reliable analysis. For in vivo applications, parenteral administration of BrdU in dosages of 5 mg/kg appears to be effective. BrdU disappears from the circulation in 30 min. Ex vivo labeling can also be achieved by brief culturing of small viable tissue specimens, immediately after removal, in medium containing 10^M BrdU. In this context the anti BrdU antibody IIB5 can be used for: 1 Radioimmunochemical determination of circulating BrdU levels after parenteral administration. 2 Detection of S-phase cells in cell suspensions by immunofluorescence microscopy. 3 Detection of S-phase cell in tissue sections by immunoperoxidase or immunofluorescence methods alone or in double immunocytochemical staining approaches. 4 Determination of the percentage of proliferating cells by flow cytometrical analysis. 5 Quantitative evaluation of the number of cells in the various phases of the cell cycle (G1, S, G2,-M) by dual parameter flow-cytometrical analysis. The antibody can be used on frozen sections and on paraffin embedded tissue after protease digestion. Dilute antibody in 0.15 M phosphate buffered saline with 1% BSA and 1% Na-azide. Advised working dilution: 1:5 - 1:10. Optimal dilution should be tested by serial dilution. Recommended for positive control: Bromodeoxyuridine labeled cells.


Ascites, 0.02% sodium azide
+4°C. Store undiluted.
For research use only.

About BrdU

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. 5-Bromodeoxycytidine is deaminated to form BrdU. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle during which DNA is replicated), substituting for thymidine during DNA replication.

Requested From: 
Date Requested: 4/24/2017

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