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Anti-BrdU Antibody (clone Bu20a, PE) LS-C204855

Catalog Size Price
LS-C204855-100 100 tst Unavailable

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100% Guaranteed
Mouse Monoclonal [clone Bu20a] (IgG1)
Flow Cytometry
PE Conjugated

Details

BrdU
Mouse
IgG1 Monoclonal [Bu20a]
Phycoerythrin
Protein A purified
Unmodified

Applications

Flow Cytometry

Specificity and Use

BrdU antibody was raised against bromodeoxyuridine conjugated to BSA.
Monoclonal antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining.
The reagent is designed for Flow Cytometry analysis of human blood cells using 10 ul reagent / 100 ul of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

PBS, 0.2% BSA, 0.02% sodium azide.
Store in the dark at 2-8 C. Do not freeze. Avoid prolonged exposure to light.
For research use only.

About BrdU

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. 5-Bromodeoxycytidine is deaminated to form BrdU. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle during which DNA is replicated), substituting for thymidine during DNA replication.

Requested From: 
Date Requested: 10/23/2017

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