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Anti-BRD4 Antibody (aa651-700) IHC-plus™ LS-B924

Note: This antibody replaces LS-C30517

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BRD4 Antibodies

Anti-BRD4 Antibody (aa651-700) IHC-plus™ LS-B924
Rabbit Polyclonal to Human BRD4
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-BRD4 Antibody (N-Terminus, APC) LS-C275695
Rabbit Polyclonal (IgG) to Human BRD4
Human
IHC, Western blot, ELISA
APC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human BRD4
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human BRD4
Rabbit
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

BRD4 antibody was raised against synthetic peptide located between aa651-700 of human BRD4 (O60885-2, NP_055114). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Lizard (100%).
LS-E14092 - Lyophilized - 100 µg - $145.00
aa651-700
Human BRD4
Immunohistochemistry: LS-B924 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B924 was determined to be 10 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl After adding water, will consist of PBS buffer with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About BRD4

O60885 NM_014299 NP_055114.1

BRD4 Antibody, Bromodomain containing 4 Antibody, Chromosome-associated protein Antibody, MCAP Antibody, HUNK1 Antibody, Protein HUNK1 Antibody, Bromodomain-containing 4 Antibody, HUNKI Antibody

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure.

Immunohistochemistry

Immunohistochemistry
Anti-BRD4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B924 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
BRD4 antibody LS-B924 Western blot of fetal thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Fetal Thymus Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/9/2016

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