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Anti-BRD3 Antibody (aa648-697) IHC-plus™ LS-B6601

Note: This antibody replaces LS-C101497

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BRD3 Antibodies

Anti-BRD3 Antibody LS-C77294
Mouse Monoclonal (IgG1) to Human BRD3
Human, Mouse, Rat
ICC, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
Anti-BRD3 Antibody (aa648-697) IHC-plus™ LS-B6601
Rabbit Polyclonal (IgG) to Human BRD3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-BRD3 Antibody (aa672-702) IHC-plus™ LS-B10710
Rabbit Polyclonal to Human BRD3
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human BRD3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human BRD3
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

BRD3 antibody was raised against synthetic peptide located between aa648-697 of human BRD3 (Q15059, NP_031397). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%); Xenopus (92%).
LS-E12206 - Lyophilized - 100 µg - $145.00
aa648-697
Human BRD3
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Reconstitute with 50ul sterile ddH2O.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About BRD3

Q15059 NM_007371 NP_031397.1

BRD3 Antibody, Bromodomain-containing 3 Antibody, Fsrg2 Antibody, KIAA0043 Antibody, ORFX Antibody, RING3-like protein Antibody, RING3L Antibody, Bromodomain containing 3 Antibody

Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). Regulates transcription of the CCND1 gene.

Immunohistochemistry

Immunohistochemistry
Anti-BRD3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6601 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
BRD3 antibody LS-B6601 Western blot of Fetal Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/7/2016

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