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Anti-BRD3 Antibody (aa647-696) LS-C110336

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

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Rabbit Polyclonal to Human BRD3
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Zebrafish
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human BRD3
Rabbit
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Bovine, Xenopus, Drosophila (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

  • IHC - Paraffin

Specificity and Use

BRD3 antibody was raised against synthetic peptide located between aa647-696 of human BRD3 (Q15059, NP_031397). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Zebrafish (100%); Bovine, Xenopus (92%); Drosophila (91%); Pig (85%); Trout (83%).
LS-E14030 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C110336. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa647-696
Human BRD3
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About BRD3

Q15059 NM_007371 NP_031397.1

BRD3 Antibody, Bromodomain-containing 3 Antibody, Fsrg2 Antibody, KIAA0043 Antibody, ORFX Antibody, RING3-like protein Antibody, RING3L Antibody, Bromodomain containing 3 Antibody

Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). Regulates transcription of the CCND1 gene.

Western blot

Western blot
BRD3 antibody LS-C110336 Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 4/22/2017

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