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Anti-BRD2 / RING3 Antibody (aa524-573) IHC-plus™ LS-B923

Note: This antibody replaces LS-C30011

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular BRD2 / RING3 Antibodies

Anti-BRD2 / RING3 Antibody (aa524-573) IHC-plus™ LS-B923
Rabbit Polyclonal to Human BRD2 / RING3
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-BRD2 / RING3 Antibody (aa348-455, Biotin) LS-C355049
Mouse Monoclonal (IgG1) to Human BRD2 / RING3
Human
Immunoprecipitation
Biotin Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human BRD2 / RING3
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human BRD2 / RING3
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

BRD2 / RING3 antibody was raised against synthetic peptide located between aa524-573 of human BRD2 (P25440, NP_005095). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum (100%); Xenopus (92%); Zebrafish (91%); Guinea pig (85%).
LS-E12112 - Lyophilized - 100 µg - $145.00
aa524-573
Human BRD2
Immunohistochemistry: LS-B923 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B923 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (1)

1
Characterization of BRD4 during Mammalian Postmeiotic Sperm Development. Bryant JM, Donahue G, Wang X, Meyer-Ficca M, Luense LJ, Weller AH, Bartolomei MS, Blobel GA, Meyer RG, Garcia BA, Berger SL. Molecular and cellular biology. Apr;35:1433-48. [Full Text Article] [PubMed:25691659]

About BRD2 / RING3

P25440 NM_005104 NP_005095.1

BRD2 Antibody, Bromodomain containing 2 Antibody, FSRG1 Antibody, KIAA9001 Antibody, NaT Antibody, RING3 Antibody, RNF3 Antibody, O27.1.1 Antibody, Bromodomain-containing 2 Antibody, D6S113E Antibody, FSH Antibody

BRD2 / RING3 is a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains.

Immunohistochemistry

Immunohistochemistry
Anti-BRD2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B923 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-BRD2 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B923 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
BRD2 antibody LS-B923 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/7/2016

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