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Anti-BRAP2 Antibody (aa67-79) IHC-plus™ LS-B3402

Note: This antibody replaces LS-C3217

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BRAP2 Antibodies

Anti-BRAP2 Antibody (aa67-79) IHC-plus™ LS-B3402
Rabbit Polyclonal to Human BRAP2
Human, Monkey
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-BRAP2 Antibody (aa66-95, APC) LS-C252663
Rabbit Polyclonal (IgG) to Human BRAP2
Human
Western blot, ELISA
APC Conjugated
Anti-BRAP2 Antibody (aa66-95, Azide-free, HRP) LS-C252666
Rabbit Polyclonal (IgG) to Human BRAP2
Human
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human BRAP2
Human, Monkey
IHC - Paraffin, ELISA
Unconjugated

Details

Human BRAP2
Rabbit
Human, Monkey (tested or 100% immunogen sequence identity)
Mouse, Dog, Horse (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • ELISA (1:1000)

Specificity and Use

BRAP2 antibody was raised against synthetic peptide from human BRAP. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse, Elephant, Panda, Dog, Horse (92%); Hamster, Rabbit, Pig (85%).
aa67-79
KLH conjugated synthetic peptide comprising amino acids 27 - 39 [AAGEMSDEEIKKT] of the human BRCA1 associated protein (BRAP) [N-terminus] protein.
Immunohistochemistry: LS-B3402 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3402 was determined to be 10 ug/ml.

Packaging

PBS, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About BRAP2

Q7Z569 NM_006768 NP_006759.3

BRAP Antibody, BRAP2 Antibody, IMP Antibody, Galectin-2-binding protein Antibody, RING finger protein 52 Antibody, RNF52 Antibody, BRCA1 associated protein Antibody, BRCA1-associated protein Antibody

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.

Immunohistochemistry

Immunohistochemistry
Anti-BRAP antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3402 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/4/2016

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