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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BRAF / B‑Raf Antibody (clone 4G6) LS‑C114548
B-Raf antibody LS-C114548 is an unconjugated mouse monoclonal antibody to B-Raf (BRAF) from human and monkey. Validated for IHC and WB.
Catalog
Size
Price
LS-C114548-100
100 µl
Unavailable

Popular BRAF / B-Raf Products

Antibody
Species: Human, Monkey, Dog, Horse
Applications: Western blot
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1627 dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3444 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3862 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
All lanes: Anti B Raf at 0.5 ug/ml. Lane 1: Rat Testis Tissue at 50 ug. Lane 2: Rat Brain Tissue at 50 ug. Lane 3: Mouse Testis Tissue at 50 ug. Lane 4: Mouse Brain Tissue at 50 ug. Lane 5: HeLa Whole Cell Lysate at 40 ug. Lane 6: Jurkat Whole Cell Lysate at 40 ug. Lane 7: MCF-7 Whole Cell Lysate at 40 ug. Lane 8: K562 Whole Cell Lysate at 40 ug. Predicted band size: 84 kD . Observed band size: 84 kD.
Species: Human, Mouse, Rat
Applications: Western blot

Product Description

B-Raf antibody LS-C114548 is an unconjugated mouse monoclonal antibody to B-Raf (BRAF) from human and monkey. Validated for IHC and WB.
About BRAF / B-Raf
BRAF / B-Raf is a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. P15056 NM_004333 NP_004324.2

BRAF Antibody, 94 kDa B-raf protein Antibody, B-raf Antibody, BRAF1 Antibody, B Raf Antibody, Proto-oncogene B-Raf Antibody, p94 Antibody, B-RAF1 Antibody, NS7 Antibody, ONCOGENE BRAF Antibody, RAFB1 Antibody


Specifications

Target
Human BRAF / B-Raf
Host
Mouse
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [4G6]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BRAF / B-Raf antibody was raised against protein expressed in 293T cell transfected with human BRAF expression vector.
Specificity
Human BRAF / B-Raf
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lymphoma tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lymphoma tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lung tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lung tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-BRAF monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-BRAF monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lymphoma tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lymphoma tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lung tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lung tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-BRAF monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-BRAF monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lymphoma tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lymphoma tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lung tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lung tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-BRAF monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-BRAF monoclonal antibody.

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lymphoma tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lymphoma tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded endometrium tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded endometrium tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded thyroid tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded lung tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded lung tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Adenocarcinoma of colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-BRAF monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-BRAF monoclonal antibody.


Requested From: 
Date Requested: 6/24/2018

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